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Novel approach for isolation and immobilization of a recombinant transaminase applying an advanced nanocomposite system

Von Wiley-VCH zur Verfügung gestellt

The increasing application of recombinant enzymes demands not only effective and sustainable fermentation, but also highly efficient downstream processing and further stabilization of the enzymes by immobilization. In this study, a novel approach for the isolation and immobilization of His-tagged transaminase from Chromobacterium violaceum (CvTA) has been developed. Simultaneous isolation and immobilization of a recombinant of CvTA was achieved by binding on silica nanoparticles (SNPs) with metal affinity linkers and additionally within poly(lactic acid) (PLA) nanofibers. The linker length and the nature of the metal ion significantly affected the enzyme binding efficiency and biocatalytic activity of CvTA-SNPs. Formation of PLA nanofibers by electrospinning technique enabled rapid embedding of CvTA-SNPs biocatalysts and ensured enhanced stability, activity. The developed advanced immobilization method reduced the time required for enzyme isolation, purification and immobilization by more than fourfold compared to classical stepwise technique.

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