ArtikelAnti‐biofilm Activity of Human Milk Oligosaccharides in Clinical Strains of Streptococcus agalactiae with Diverse Capsular and Sequence Typesvon Rebecca E. et al. · 14.03.2023 Shooting down a film: Purified human milk oligosaccharides have potent anti-biofilm activity against diverse colonizing and invasive clinical isolates of Group B Streptococcus. HMOs can inhibit and dismantle biofilm in both invasive and colonizing s...
ArtikelAcyltransferase Domain Exchange between Two Independent Type I Polyketide Synthases in the Same Producer Strain of Macrolide Antibioticsvon Fumitaka Kudo et al. · 14.03.2023 Fair exchange: AT domain-swapping between two independent PKSs in the same producer strain resulted in the production of two new macrolide antibiotics without supply problems of the unique extender units or loss of cytotoxic activity against HeLa ce...
ArtikelBiosynthesis of the Isocoumarin Derivatives Fusamarins is Mediated by the PKS8 Gene Cluster in Fusariumvon Anna K. et al. · 14.03.2023 Fusaria are plant-pathogenic fungi infecting several staple crops, and accumulating often toxic compounds during infection and hence threaten food security worldwide. The full chemical potential of these notorious pathogens is still elusive. Here, w...
ArtikelIdentification of 2‐Methylthio‐methylenethio‐N6‐(cis‐4‐hydroxyisopentenyl)adenosine (msms2io6A37) as a Novel Modification at Adenosine 37 of tRNAs from Salmonella typhimuriumvon Marouane Libiad et al. · 14.03.2023 New modification in an old pathway: Using high-resolution mass spectrometry, we report msms2io6A as a novel derivative of the ms2io6A biosynthesis pathway of tRNAs from S. typhimurium. This new redox hydroxylation reaction depends on the activity of...
ArtikelNutlin‐3a‐aa: Improving the Bioactivity of a p53/MDM2 Interaction Inhibitor by Introducing a Solvent‐Exposed Methylene Groupvon Florian Nietzold et al. · 14.03.2023 Look away. Modification of the p53/MDM2 inhibitor Nutlin-3a with an exocyclic methylene group generated the more potent inhibitor Nutlin-3a-aa. X-ray analysis of MDM2-bound Nutlin-3a-aa indicated that the exocyclic methylene group of Nutlin-3a-aa fa...
ArtikelExploring the Flexibility of the Glycopeptide Antibiotic Crosslinking Cascade for Extended Peptide Backbonesvon Y. T. et al. · 14.03.2023 Glycopeptide antibiotic (GPA) crosslinking is performed by cytochrome P450 (Oxy) enzymes and is essential for antibiotic activity. We show that these Oxy enzymes are tolerant of extension at the N but not the C termini of their peptide substrates. A...
ArtikelBiochemical Analysis Leads to Improved Orthogonal Bioluminescent Toolsvon Sierra J. et al. · 14.03.2023 If you can stand the heat: The substrate selectivity and thermostability of mutant luciferases were analyzed by using a biochemical approach. Mutants derived from two popular luciferases, Pecan and Cashew, were made and assessed to find key residues...
ArtikelFront Cover: Biosynthesis of the Isocoumarin Derivatives Fusamarins is Mediated by the PKS8 Gene Cluster in Fusarium (ChemBioChem 6/2023)von Anna K. et al. · 14.03.2023 Fusaria are plant-pathogens known to produce various secondary metabolites (SMs). Yet, the majority of them is still cryptic due to low production levels in axenic culture, and even less are assigned to their respective biosynthetic gene cluster (BG...
ArtikelCover Feature: Exploring the Flexibility of the Glycopeptide Antibiotic Crosslinking Cascade for Extended Peptide Backbones (ChemBioChem 6/2023)von Y. T. et al. · 14.03.2023 Glycopeptide antibiotic (GPA) crosslinking is performed by cytochrome P450 (Oxy) enzymes and is essential for antibiotic activity. Here, we symbolise the process by which we screen for Oxy activity toward altered peptide substrates, which revealed t...
ArtikelLive‐Cell Glycocalyx Engineeringvon Yuxin Li et al. · 14.03.2023 New advances in developing tools to engineer surface glycocalyx on live cells and donor organs have paved a transformative road towards glycotherapy for anticancer, anti-inflammation, and anti-autoimmune agents, as well as transplantation and other ...
ArtikelSynthetic Multienzyme Assemblies for Natural Product Biosynthesisvon Min Liu et al. · 14.03.2023 Constructing multienzyme complexes has become an effective way to optimize metabolic flux and increase the titer of natural products in metabolic engineering recently. In this review, we summarize strategies for constructing synthetic multienzyme co...
ArtikelTowards Understanding the Molecular Mechanisms of Immunogenic Cell Deathvon Xiaolin Xiong et al. · 14.03.2023 Only a few proteins are known to be involved in drug-triggered ICD effects, some are related to ER stress and others imply new modes of action. Here, the triggering mechanisms underlying drug-induced immunogenic cell death along with the main biotar...
ArtikelDesign of Artificial Enzymes: Insights into Protein Scaffoldsvon Stefanie Hanreich et al. · 14.03.2023 The design of artificial enzymes has emerged as a promising tool for the generation of potent biocatalysts able to promote new-to-nature reactions. This review aims to give a general overview of suitable protein scaffolds, that could be functionaliz...
ArtikelMissing Pieces in Structure Puzzles: How Hyperpolarized NMR Spectroscopy Can Complement Structural Biology and Biochemistryvon Mattia Negroni et al. · 14.03.2023 Accessing inaccessible spaces: The inclusion of hyperpolarized NMR in established experimental methods can provide complementary information about formerly inaccessible conformational spaces. Structural models under physiological conditions or of sh...
ArtikelUnderstanding the Interactions of Guanine Quadruplexes with Peptides as Novel Strategies for Diagnosis or Tuning Biological Functionsvon Tom Miclot et al. · 14.03.2023 G4s for control and diagnosis: We review the study of the specific interactions between G4 (either DNA or RNA) and small synthetic and natural peptides. In particular, we highlight the complementary role of spectroscopic techniques and molecular mod...
ArtikelStairway to Stereoisomers: Engineering Short‐ and Medium‐Chain Ketoreductases To Produce Chiral Alcoholsvon Anirudh P. · 14.03.2023 Most chiral alcohols are generated in labs by using short- and medium-chain dehydrogenase/reductases. In the natural world, they produce biopolymers, active pharmaceutical intermediates and bioactive compounds. Different engineering and catalysis me...
ArtikelReversible Photocontrol of Microtubule Stability by Spiropyran‐Conjugated Tau‐Derived Peptidesvon Hiroshi Inaba et al. · 20.03.2023Spatiotemporal modulation of microtubules by light has become an important aspect of the biological and nanotechnological applications of microtubules. We previously developed a Tau-derived peptide as a binding unit to the inside of microtubules. Her...
ArtikelGlycosphingolipid and Glycosylphosphatidylinositol Affect Each Other in and on the Cellvon Zhongwu Guo · 20.03.2023Glycosphingolipid (GSL) and glycosylphosphatidylinositol (GPI) are the two major glycolipids expressed by eukaryotic cells, and their metabolisms share the same machineries. Moreover, both GSLs and GPI-anchored proteins (GPI-APs) are localized in the...
ArtikelScientific Fluency as the Greatest Strength of Chemical Biologistsvon Nicholas A. et al. · 17.03.2023 Eclectic: Chemical biology has become an influential discipline thanks to synergistic relationships between students from diverse scientific backgrounds. Our field relies on the fluency of chemical biologists in traversing disciplines. In this colle...
ArtikelSystematic investigation of metabolic oligosaccharide engineering efficiency in intestinal cells using a dibenzocyclooctyne‐monosaccharide conjugatevon Yuen et al. · 16.03.2023Metabolic oligosaccharide engineering (MOE) of cells with synthetic monosaccharides can introduce functionality to the glycans of cell membranes. Unnatural sugars (e.g., peracetylated mannose-azide) can be expressed on the cell surface with the azide...
ArtikelDifferential Chemoproteomics Reveals MARK2/3 as Cell Migration‐Relevant Targets of the ALK Inhibitor Brigatinibvon Qianqian Hu et al. · 16.03.2023Metastasis poses a major challenge in cancer management, including EML4-ALK-rearranged non-small cell lung cancer (NSCLC). As cell migration is a critical step during metastasis, we assessed the anti-migratory activities of several clinical ALK inhib...
ArtikelProtein Ligation‐assisted Reconstitution of Split HRP Fragments for Facile Production of HRP fusion Proteins in E. colivon Seungnyeong Heo et al. · 16.03.2023Horseradish peroxidase (HRP) is a pivotal biocatalyst for biosensor development and fine chemical synthesis. HRP proteins are mostly extracted and purified from the roots of horseradish because the solubility and productivity of recombinant HRP in ba...
ArtikelProtein Backbone Alteration in Non‐hairpin β‐Turns: Impacts on Tertiary Folded Structure and Folded Stabilityvon Thomas et al. · 15.03.2023The importance of β-turns to protein folding has motivated extensive efforts to stabilize the motif with non-canonical backbone connectivity. Prior work has focused almost exclusively on turns between strands in a β-sheet (i.e., hairpins). Turns in o...
ArtikelTaking Synthetic Biology to the Seas – From Blue Chassis Organisms to Marine Aquaformingvon Lennart Schada · 15.03.2023Oceans cover 71% of Earth’s surface and are home to hundreds of thousands of species, many of which are microbial. Knowledge about marine microbes has strongly increased in the past decades due to global sampling expeditions and hundreds of detailed ...
ArtikelDiscovery and Characterization of a Baeyer‐Villiger Monooxygenase using Sequence Similarity Network Analysisvon Thaleia Sakoleva et al. · 15.03.2023Baeyer-Villiger monooxygenases (BVMOs) are important flavin-dependent enzymes which perform oxygen insertion reactions leading to valuable products. As reported in many studies, BVMOs are usually unstable during application, preventing a wider usage ...
ArtikelConstraining and modifying peptides using Pd‐mediated cysteine allylationvon Julia Kriegesmann et al. · 14.03.2023Over the past decades, several strategies for inducing and stabilizing secondary structure formation in peptides have been developed to increase their proteolytic stability and their binding affinity to specific interaction partners. Here, we report ...
ArtikelRecent Advances in Metal Complexes for Antimicrobial Photodynamic Therapyvon Thomas et al. · 14.03.2023Antimicrobial resistance (AMR) is a growing global problem with more than 1 million deaths due to AMR infection in 2019 alone. New and innovative therapeutics are required to overcome this challenge. Antimicrobial photodynamic therapy (aPDT) is a rap...
ArtikelStructural basis for activation of cobalt‐carbon bond and control of adenosyl radical in coenzyme B12 catalysisvon Naoki Shibata et al. · 14.03.2023Adenosylcobalamin (AdoCbl) or coenzyme B12 is a naturally occurring organometallic compound that serves as a cofactor for enzymes that catalyze intramolecular group-transfer reactions and ribonucleotide reduction in a wide variety of organisms from b...
ArtikelComputationally supported inversion of ketoreductase stereoselectivityvon Estela Delgado-Arciniega et al. · 14.03.2023Whereas directed evolution and rational design by structural inspection are established tools for enzyme redesign, computational methods are less mature but have the potential to predict small sets of mutants with desired properties without laborator...
ArtikelIncorporating a Polyethyleneglycol Linker to Enhance the Hydrophilicity of Mitochondria‐Targeted Triphenylphosphonium Constructsvon Shinpei Uno et al. · 14.03.2023The targeting of bioactive molecules and probes to mitochondria can be achieved by coupling to the lipophilic triphenyl phosphonium (TPP) cation, which accumulates several hundred-fold within mitochondria in response to the mitochondrial membrane pot...
ArtikelTwo‐Step Validation Approach for Tools to Study the DNA Repair Enzyme SNM1Avon Ellen Fay et al. · 14.03.2023We report a two-step validation approach to evaluate the suitability of metal-binding groups for targeting DNA damage repair metalloenzymes, using model enzyme SNM1A. A fragment-based screening approach was first used to identify metal-binding fragme...
ArtikelEtoposide‐Entrapped Progesterone‐Cationic Lipid Nanoaggregates as Selective Therapeutics against Etoposide‐Resistant Colorectal Cancer Cellsvon Mohammed et al. · 11.03.2023Drug resistance majorly impacts the treatment of several cancers. This is mainly due to the overexpression of cellular drug efflux proteins. Hence, drug delivery systems that can avoid this resistance are needed. We report PR10, a progesterone-catio...
ArtikelDetection and Imaging of Active Substances in Early Atherosclerotic Lesions using Fluorescent Probesvon Jin Li et al. · 11.03.2023Atherosclerosis (AS) is a vascular disease caused by chronic inflammation and lipids, which is the main cause of myocardial infarction, stroke and other cardiovascular diseases. Atherosclerosis is often difficult to detect in early stages due to the ...
ArtikelAsymmetric Bio‐epoxidation of Unactivated Alkenesvon Jiajing Li et al. · 10.03.2023 The asymmetric epoxidation reactions of a variety of unactivated alkenes, including aliphatic chain olefins, cyclic olefins, and aromatic olefins, have been widely studied. This review lists three main types of engineered enzymes for the epoxidation...
ArtikelWhole‐Cell MALDI‐ToF MS Coupled with Untargeted Metabolomics Facilitates Investigations of Microbial Chemical Interactionsvon Nicole Aiosa et al. · 10.03.2023 Screening microbial chemical interactions: A high-throughput whole-cell MALDI method has been developed for screening chemical interactions between commensal and pathogenic bacteria. This enabled identification of natural product compounds produced ...
ArtikelMesoporous Silica Nanoparticles: Drug Delivery Vehicles for Antidiabetic Moleculesvon Sanjib Sarkar et al. · 10.03.2023 Mesoporous silica nanoparticles (MSNs) are promising nanomaterials for use in biomedical applications. MSN-based nanocomposites have been used to deliver antidiabetic drugs like insulin. The functionalisation properties of MSNs make them capable of ...
ArtikelMetabolomics Reveals a “Trimeric” γ‐Actinorhodin from Streptomyces coelicolor M145von Andrew P. et al. · 10.03.2023 Trimeric actinorhodin discovery: The discovery of θ-actinorhodin was enabled by differing culture conditions of Streptomyces coelicolor and the use of high-resolution LC–MS/MS to perform extensive metabolomics analysis. Abstract Streptomyces coeli...
ArtikelUnique Crosslinking Properties of Psoralen‐conjugated Oligonucleotides developed by Novel Psoralen N‐Hydroxysuccinimide Estersvon Juki Nakao et al. · 10.03.2023Psoralens and their derivatives, such as trioxsalen, have unique crosslinking features to DNA. However, psoralen monomers do not have sequence-specific crosslinking ability with the target DNA. With the development of psoralen-conjugated oligonucleot...
ArtikelMapping the chemical space of active‐site targeted covalent ligands for protein tyrosine phosphatasesvon Suk ho et al. · 09.03.2023Protein tyrosine phosphatases (PTPs) are an important class of enzymes that modulate essential cellular processes through protein dephosphorylation and are dysregulated in various disease states. There is demand for new compounds that target the acti...
ArtikelDerivatization of Mirror‐image L‐Nucleic Acids with 2'‐OMe Modification for Thermal and Structural Stabilizationvon Yuliya Dantsu et al. · 09.03.2023To further expand the functionality and enhance the stability of mirror-image nucleic acid as advanced agent for basic research and therapeutic design, we have synthesized the 2'-deoxy2'-methoxy-L-uridine phosphoramidites and incorporate it into L-DN...
ArtikelAltering the Modular Architecture of Galectins Affects its Binding with Synthetic α‐Dystroglycan O‐Mannosylated Core M1 Glycoconjugates In situvon Lareno et al. · 09.03.2023The multifunctionality of galectins helps regulate a broad range of fundamental cellular processes via cis-binding and trans-bridging activities and has gained widespread attention with respect to the importance of the natural specificity/selectivity...
ArtikelChemical Biology Approaches Toward Precise Structure Control of IgG‐based Antibody‐Oligonucleotide Conjugatesvon Lin Shen et al. · 09.03.2023Antibody-oligonucleotide conjugates (AOCs) are important tools for drug development and biochemical analysis. However, the structural heterogeneity of AOCs synthesized through conventional coupling methods raises reproducibility and safety concerns i...
ArtikelThiophene‐based Ligands: Design, Synthesis and Their Utilization for Optical Assignment of Polymorphic Disease Associated Protein Aggregatesvon Linnea Björk et al. · 09.03.2023The development of ligands for detecting protein aggregates is of great interest, since these aggregated proteinaceous species are the pathological hallmarks of several devastating diseases, including Alzheimer´s disease. In this regard, thiophene-ba...
ArtikelChemoenzymatic Synthesis of Glycopeptides to Explore the Role of Mucin 1 Glycosylation in Cell Adhesionvon Claudia Bello et al. · 09.03.2023Posttranslational modifications affect protein biology in physiological and pathological conditions. Efficient methods for the preparation of peptides and proteins carrying defined, homogeneous modifications are fundamental tools for investigating th...
ArtikelBioinformatic analysis of immobilized enzymes: towards a better understanding of the guiding forcesvon David Roura et al. · 09.03.2023Protein bioinformatics has been applied to a myriad of opportunities in biocatalysis, from enzyme engineering to enzyme discovery, but its application in enzyme immobilization is still very limited. Enzyme immobilization brings clear advantages form ...
ArtikelMechanistic Insights into the Activities of Major Families of Enzymes in Bacterial Peptidoglycan Assembly and Breakdownvon Jeric Mun et al. · 09.03.2023 Bacteria encode multiple enzymes to build and break down the essential peptidoglycan polymer. We discuss recent biochemical characterisations of major peptidoglycan enzymes that elucidate their activities, substrate preferences, regulation and poten...
ArtikelAsymmetric Ene‐Reduction by F420‐Dependent Oxidoreductases B (FDOR‐B) from Mycobacterium smegmatisvon Suk Woo et al. · 09.03.2023 The first investigation of asymmetric ene-reductions by FDOR-Bs and exploration of additional ene-reductase candidates from FDOR family will advance our understanding of FDORs as industrial biocatalysts. Due to their presence in a greater variety of...
ArtikelReplication Protein A Enhances Kinetics of Uracil DNA Glycosylase on ssDNA and Across DNA Junctions: Explored with a DNA Repair Complex Produced with SpyCatcher/SpyTag Ligationvon Sharon et al. · 08.03.2023DNA repair proteins participate in extensive protein-protein interactions that promote the formation of DNA repair complexes. To understand how complex formation affects protein function during base excision repair, we tested SpyCatcher/SpyTag ligati...
ArtikelBioorthogonal Peptide Enrichment From Complex Samples Using a Rink‐Amide‐Based Catch‐and‐Release Strategyvon Tyrza van et al. · 07.03.2023Uptake and processing of antigens by antigen presenting cells (APCs) is a key step in the initiation of the adaptive immune response. Studying these processes is complex as the identification of low abundant exogenous antigens from complex cell extra...
ArtikelSynthesis and Structural Investigation of a Foldamer based Iodine‐Supported Artificial Glycosidasesvon Olamilekan et al. · 03.03.2023Glycosidases are a type of enzymes that hydrolytically cleave the carbohydrate and form glycans for biologically important processes. The inadequacy of glycosidases or their genetic abnormalities is responsible for various diseases. Thus, the develop...
ArtikelCapturing covalent catalytic intermediates by enzyme mutants: recent advances in methodologies and applicationsvon Yongchao Wang et al. · 03.03.2023Covalent catalytic intermediates provide valuable information to reveal the catalytic mechanism, probe the enzyme activity and identify substrate specificity. However, naturally formed covalent intermediates are too rapidly degraded for general biolo...
ArtikelResorc[4]arene modifiers for supramolecular site‐directed immobilization of antibodies on multi‐walled carbon nanotubesvon Francesca Polli et al. · 03.03.2023One of the main problems in developing immunosensors featuring carbon nanotubes (CNTs) is immobilizing antibodies (Abs) onto the CNT surface to afford selective binding to target antigens (Ags). In this work, we developed a practical supramolecular A...
ArtikelpH‐Responsive and Reversible A‐motif Based DNA Hydrogel: Synthesis and Biosensing Applicationvon Vinod Morya et al. · 02.03.2023We report an A-motif functional DNA hydrogel that does not require any sequence design. A-motif DNA is a non-canonical parallel DNA duplex structure comprises homopolymeric deoxyadenosines (poly-dA) strands which undergo conformational changes from s...
ArtikelNovel Approach for the Isolation and Immobilization of a Recombinant Transaminase: Applying an Advanced Nanocomposite Systemvon Gábor Koplányi et al. · 02.03.2023 In one go: Combining nanoparticles with affinity tags and polymeric nanofibers provides an advanced nanocomposite that allows the isolation and immobilization a recombinant enzyme. The developed method reduced the time and cost required for enzyme i...
ArtikelModulation of Aβ42 Aggregation Kinetics and Pathway by Low‐Molecular‐Weight Inhibitorsvon Marie‐Theres Hutchison et al. · 02.03.2023 The extraneuronal deposition of amyloid-β 42 (Aβ42) is a hallmark of Alzheimer's disease. Here, we investigate the effect of modulators and inhibitors on early-stage Aβ42 aggregation. A differential impact was observed in terms of monomer consumptio...
ArtikelAdvances in synthetic biology of fungi and contributions to the discovery of new moleculesvon Vito Valiante · 02.03.2023The sequencing of fungal genomes is becoming increasingly accessible, with a wealth of data already available. In parallel, the prediction of putative biosynthetic pathways responsible for the synthesis of potential new natural products is also incre...
ArtikelTracking the Message: Applying Single Molecule Localization Microscopy to Cellular RNA Imagingvon Benoît Arnould et al. · 01.03.2023RNA function is increasingly appreciated to be more complex than merely communicating between DNA sequence and protein structure. RNA localization has emerged as a key contributor to the intricate roles RNA plays in the cell, and the link between dys...
ArtikelControlled Membrane Transport in Polymeric Biomimetic Nanoreactorsvon Shoupeng Cao et al. · 01.03.2023 Controlling membrane permeability: Polymeric biomimetic nanoreactors are compartmentalized catalytic systems with applications in nanomedicine and biomimicry. Membrane permeability of biomimetic nanoreactors is a key feature that determines their fu...
ArtikelA Nucleophilic Activity‐Based Probe Enables Profiling of PLP‐Dependent Enzymesvon Scott I. et al. · 01.03.2023 The first activity-based probe for PLP-dependent enzymes is reported. The trifunctional probe contains an N-hydroxyalanine warhead, a linear diazirine, and an alkyne to enable enrichment. The essential protein LysA was identified as the primary targ...
ArtikelType II Polyketide Synthases: A Bioinformatics‐Driven Approachvon Shengling Xie et al. · 01.03.2023Bioinformatics has become an indispensable tool for natural products research in the genomic era. One of the key challenges is to accurately convert sequence data of a biosynthetic gene cluster into chemical information such as the enzymatic function...
ArtikelBioorthogonal Probes for Analyzing Non‐Enzymatic Post‐Translational Modificationsvon Zhentao Shao et al. · 01.03.2023Non-enzymatic post-translational modifications (nPTMs) have been proposed as indicators of cellular stresses and diseases. Unfortunately, direct assessment of nPTMs in native environment is extremely challenging due to the heterogeneity of adducts an...
ArtikelOligonucleotides Containing C5‐Propynyl Modified Arabinonucleic Acids: Synthesis, Biophysical and Antisense Propertiesvon Alexander Pontarelli et al. · 01.03.2023The introduction of chemical modifications on the nucleic acid scaffold has allowed for the progress of antisense oligonucleotides (ASOs) in the clinic for the treatment of a variety of disorders. In contribution to the repertoire of gene-silencing n...
ArtikelAn easily accessible NIR‐Absorbing tetraimide dye and its biotherapeutics based photothermal and photodynamic therapyvon Dasari Srideep et al. · 28.02.2023Organic p-systems with strong absorption in the near-infrared (NIR) region are promising candidates for photothermal therapy (PTT) and photodynamic therapy (PDT). However, the synthesis of NIR p-systems involves several steps and many of them displa...
ArtikelEFMC: Trends in Medicinal Chemistry and Chemical Biologyvon Yves P. et al. · 28.02.2023 A wealth of opportunities: Novel tools for chemical biology including chemical inducers of proximity, designer probes or RNA-targeting agents enable innovative tactics for therapeutic approaches. This article explores recent developments combining t...
ArtikelStudying Reversible Protein Post‐translational Modification through Co‐translational Modificationvon Dan Wu et al. · 28.02.2023 Chemical biology tools have transformed the field of epigenetics. In this Concept, genetically modified organisms for epigenetic studies are discussed along with how genetic code expansion technology is used to introduce particular co-translationall...
ArtikelSelf‐Assembled DNA Nanocages Promote Cell Migration and Differentiation of Human Umbilical Vein Endothelial Cells von Anjali Rajwar et al. · 28.02.2023 Shaping up: DNA nanocages get taken up into endothelial cells where they stimulate endothelial cell differentiation and migration. This could have potential application in angiogenesis. Moreover, changing the topology of the nanocage can have an inf...
ArtikelGenetic Encoding of a Photocaged Histidine for Light‐Control of Protein Activityvon Jenny W. et al. · 28.02.2023 Controlling histidine-containing proteins: The addition of photocaged histidine to the genetic code enables light-based control of protein function in bacteria and mammalian cells. Abstract The use of light to control protein function is a critica...
ArtikelCo‐translational Installation of Posttranslational Modifications by Non‐canonical Amino Acid Mutagenesisvon Wei Niu et al. · 28.02.2023Protein posttranslational modifications (PTMs) play critical roles in regulating cellular activities. Here we provide a survey of genetic code expansion (GCE) methods that were applied in the co-translational installation and studies of PTMs through...
ArtikelMetal Complexes and Nanoparticles for Photoacoustic Imagingvon Axel Steinbrueck et al. · 28.02.2023Clinical imaging techniques are widely used to detect, locate, and track the growth or shrinkage of cancerous tumors. Although these techniques have shown impressive results, they often come with health risks due to the use of toxic contrast agents o...
ArtikelIsotope‐guided metabolomics reveals divergent incorporation of valine into different flavor precursor classes in chivesvon Ricarda Freke et al. · 28.02.2023Plants of the genus Allium such as chives, onions or garlic produce S-alk(en)yl cysteine sulfoxides as flavor precursors. Two major representatives are S-propenyl cysteine sulfoxide (isoalliin) and S-propyl cysteine sulfoxide (propiin), which only di...
ArtikelStrategies for synthesizing and enhancing the immune response of cancer vaccines based on MUC1 glycopeptide antigensvon Lixin Yin et al. · 24.02.2023Cancer vaccines are based on a vaccinology strategy whereby the patient’s immune system is harnessed to induce a specific immune response to kill cancer cells and comprises two categories: prophylactic and therapeutic. Glycoprotein mucin 1 (MUC1), wh...
ArtikelHaloTag‐based reporters for fluorescence imaging and biosensingvon Alexander Cook et al. · 23.02.2023Visualizing the structure and dynamics of biomolecules is critical to understand biological function, and requires methods to fluorescently label targets of interest in their cellular context. Self-labelling proteins, which combine a genetically enco...
ArtikelRapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters utilizing Microbial Aromatic Acid CoA Ligasesvon Debayan Chaudhury et al. · 23.02.2023Chemically labile ester linkages can be introduced into lignin by incorporation of monolignol conjugates, which are synthesized in planta by acyltransferases that use a coenzyme A (CoA) thioester donor and a nucleophilic monolignol alcohol acceptor. ...
ArtikelLysosomes targeting pH activable imaging‐guided photodynamic agentsvon Carlotta Figliola et al. · 23.02.2023Photodynamic therapy (PDT) is a photochemistry-based medical treatment combining light at a specific wavelength and a photosensitizer (PS) in the presence of oxygen. Application of PDT as a conventional treatment is limited and clearly the approval i...
ArtikelCell wall‐denaturing molecules for plant gene modificationvon Risa Naka et al. · 22.02.2023Zwitterionic molecules, such as zwitterionic liquids (ZILs) and polypeptides (ZIPs), are attracting attention for application in new methods that can be used to loosen tight cell wall networks in a biocompatible manner. These novel methods can enhanc...
ArtikelA Detailed View on Geosmin Biosynthesisvon Houchao Xu et al. · 21.02.2023The bacterial geosmin synthase is a fascinating bifunctional enzyme that has been discovered almost two decades ago. Several aspects of the cyclisation mechanism from FPP to geosmin are known, but a detailed picture of the stereochemical course of th...
ArtikelAdvances in Multifunctional Chemotherapeutic Prodrugs for Near‐infrared Fluorescence Imaging‐guided Therapyvon Jiayu Zeng et al. · 18.02.2023Conventional chemotherapy (CT) is associated with severe side effects and inducible resistance, making it difficult to meet clinical requirements, forcing the development of new multifunctional prodrugs for precision medicine. In recent decades, rese...
ArtikelRecent Advances in Protein‐Specific Glycan Imaging and Editingvon Xiaocui Zheng et al. · 17.02.2023Glycosylation in live cell and animal modulate a constellation of biological functions. The advent of Chemical Biology has revolutionized the analysis and tailoring of glycans, by introducing myriads of glycan engineering methods. However, the ideal ...
ArtikelFusion Enzymes Involved in Biosynthetic Tailoring Reactions in Fungivon Jia-jing Liang et al. · 17.02.2023Enzyme fusion, the fusion of enzymes with different domains to a single protein, has been widely recognized as a promising strategy in the development of biocatalysts. Nature has evolved gene fusion to combine different catalytic enzymes to function ...
ArtikelRational Design of a Highly Specific Prolyl Endopeptidase To Activate the Antihypertensive Effect of Peptidesvon Mingzhe Ma et al. · 16.02.2023 Eat and relax: By using an energy-optimized rational design strategy, the antihypertensive properties of food-derived proteins have been activated through hydrolysis with highly specific proline endopeptidases. Abstract Enzymatic hydrolysis of foo...
ArtikelCannabidiol‐loaded poly lactic‐co‐glycolic acid nanoparticles with improved bioavailability as a potential for osteoarthritis therapeuticvon Zhiqiang Jin et al. · 16.02.2023Cannabidiol (CBD) is a non-intoxicating cannabinoid from cannabis sativa that has demonstrated efficacious against inflammation, which can be considered as a potential drug for arthritis treatment. However, the poor solubility and low bioavailability...
ArtikelInvestigation of Sequence‐Penetration Relationships of Antisense Oligonucleotidesvon Nefeli Batistatou et al. · 15.02.2023A major limitation for the development of more effective oligonucleotide therapeutics has been a lack of understanding of their penetration into the cytosol. While prior work has shown how backbone modifications affect cytosolic penetration, it is un...
ArtikelExploring the effects of mutagenesis on FusionRed using excited state QM/MM dynamics and classical force field simulationsvon Ashlyn et al. · 14.02.2023Fluorescent proteins (FPs) are a powerful tool for examining tissues, cells, and subcellular components in vivo and in vitro. FusionRed is a particular FP variant mutated from mKate2 that, in addition to lower cytotoxicity and aggregation rates, has ...
ArtikelQuality Data from Messy Spectra: How Isometric Points Increase Information Content in Highly Overlapping Spectravon Felix Kaspar · 14.02.2023 Spectroscopic reaction monitoring often yields complex and highly overlapping data, which can easily be untangled with the help of isometric points. This allows one to quantitatively follow the progress of chemical transformations from messy spectro...
ArtikelPeptide‐based nanoarchitectonics for the treatment of liver fibrosisvon Bowen Li et al. · 14.02.2023Liver fibrosis is a process of excessive accumulation of extracellular matrix caused by liver injury. Liver fibrosis can progress to cirrhosis or even liver cancer if not intervened properly. Till now, no effective therapeutic drugs have been clinica...
ArtikelIntracellular Delivery of mRNA for Cell‐Selective CRISPR/Cas9 Genome Editing using Lipid Nanoparticlesvon Tianyu Ma et al. · 13.02.2023Messenger RNA (mRNA) is being used as part of an emerging class of biotherapeutics with great promise for preventing and treating a wide range of diseases, as well as encoding programmable nucleases for genome editing. However, the low stability, imm...
ArtikelDeacetylation of arabinosylated xylosyl residues related to plant xylan: significant differences between xylan deacetylases classified into various carbohydrate esterase familiesvon Maroš Bella et al. · 13.02.2023A chemical synthesis of two novel phenyl glycosides of trisaccharides related to acetylarabinoxylan is described. The trisaccharides bear acetyl and arabinofuranosyl moieties at the non-reducing-end xylopyranosyl residue, which is substituted at posi...
ArtikelNew Biocompatible β‐Phosphorylated Linear Nitrones Targeting Mitochondria: Protective Effect in Apoptotic Cellsvon Marcel Culcasi et al. · 13.02.2023Mitochondria, an essential organelle involved in cellular respiration, energy production, and cell death, is the main cellular source of reactive oxygen species (ROS), including superoxide. Mitochondrial diseases resulting from uncontrolled/excess RO...
ArtikelPolypyridyl Co(II)‐Curcumin Complexes as Photo‐activated Anticancer and Antibacterial Agentsvon Ashish et al. · 10.02.2023Four new Co(II) complexes viz., [Co(bpy)2(acac)]Cl (1), [Co(phen)2(acac)]Cl (2), [Co(bpy)2(cur)]Cl (3), [Co(phen)2(cur)]Cl (4), where bpy = 2,2’-bipyridine (1 and 3), phen = 1,10-phenanthroline (2 and 4), acac = acetylacetonate (1 and 2), cur = curcu...
ArtikelPhotocatalytic proximity labeling for profiling the subcellular organization of biomoleculesvon Yuxin Fang et al. · 10.02.2023Investigating the subcellular organization of biomolecules is important for understanding their biological functions. Over the past decade, proximity-dependent labeling methods have emerged as powerful tools for mapping biomolecules in their native c...
ArtikelROS‐Triggered Gel–Sol Transition and Kinetics‐Controlled Cargo Release by Methionine‐Containing Peptidesvon Yoshika Hara et al. · 09.02.2023Gel–sol transition of self-assembling peptides is a useful switch for environment-dependent drug release. For their applications, kinetics control of the responses is important for matching the releasing velocity to the target biological events. Here...
ArtikelEffects of Epitranscriptomic RNA Modifications on the Catalytic Activity of SARS‐CoV‐2 Replication Complexvon Alexander Apostle et al. · 08.02.2023SARS-CoV-2 causes individualized symptoms. Many reasons have been given. We propose that an individual’s epitranscriptomic system could be responsible as well. The viral RNA genome can be subject to epitranscriptomic modifications, the modifications ...
ArtikelCross‐linked enzyme aggregates of fusion enzymes in microaqueous organic mediavon Guillem Vernet et al. · 07.02.2023Baeyer-Villiger Monooxygenases (BVMOs) are attractive for selectively oxidizing various ketones using oxygen into valuable esters and lactones. However, the application of BVMOs is restrained by cofactor-dependency and enzyme instability combined wit...
ArtikelIntroducing Nanozymes: New Horizons in Periodontal and Dental Implant Carevon Bijun Zhu et al. · 03.02.2023 Nanozymes mimic enzyme functions for use in periodontology and implantology, as illustrated on the cover. Periodontal and peri-implant diseases are important diseases worldwide. As multifunctional nanomaterials with enzyme-mimicking activities, nano...
ArtikelA Chimeric Peptide Inhibits Red Blood Cell Invasion by Plasmodium falciparum with Hundredfold Increased Efficacy von Jamsad Mannuthodikayil et al. · 31.01.2023 Inhibition of malaria parasite entry into red blood cells by a rationally designed chimeric peptide molecule that exhibits high affinity to parasite protein receptor (AMA1) displayed on the merozoite surface and outcompetes the natural ligand for th...
