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Suitability of Sodium Pyruvate as Decomposer of Carried‐over Liquid Hydrogen Peroxide
Von Wiley-VCH zur Verfügung gestellt
The concentration of sodium pyruvate in growth media by using the most-probable number method to decompose carried-over liquid H2O2 without negative influences on correct enumeration of Geobacillus stearothermophilus spores is evaluated. An equivalent molar ratio of sodium pyruvate and H2O2 was verified to be sufficient for a complete decomposition.
Abstract
The aim of the present study was to evaluate the concentration of sodium pyruvate in growth media by using the most-probable number method to decompose carried-over liquid hydrogen peroxide (H2O2) without negative influences on correct enumeration of Geobacillus stearothermophilus spores. An equivalent molar ratio of sodium pyruvate and H2O2 was verified to be sufficient for a complete decomposition. The results showed that by exposition of G. stearothermophilus spores in different liquid hydrogen peroxide and by carry-over of concentrations ranging from 3.56 · 10−6 up to 1.69 · 10−4 mol, a sodium pyruvate concentration of 0.05 mol L−1 in growth media was most efficient for the recovery of spores.
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