Fabrication of Carbon Paste Sensor Activated with Electropolymerized DL‐Phenylalanine for the analysis of Levofloxacin

This study illustrates the utility and efficacy of an electropolymerised DLP deposited carbon paste electrode which is easy to use and sensitive for the detection of LF compounds that are significant for medicines. SEM, EIS, and CV techniques were used to characterize the prepared electrodes. At the surface of the modified electrode with a reduced LOD, the LF molecule exhibits an effective electrochemical response. The LF in food samples is detected with high recovery by the electropolymerised DLP placed carbon paste electrode.

Abstract

Voltammetric analysis of Levofloxacin (LF) using an electrochemically polymerized composite of DL-phenylalanine (DLP) alongside a bare carbon paste electrode. The constructed electrodes are polymerized DL-phenylalanine modified carbon paste electrodes P(DLP/MCPE) and bare carbon paste electrodes (BCPE). Cyclic voltammetry (CV), differential pulse voltammetry (DPV), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and other electrochemical techniques used for characterization of P(DLP/MCPE) and BCPE in 0.2 M phosphate buffer solution (PBS) with pH 4.5 were conformed using the P(DLP/MCPE), and it shows the highest current responses compared to the BCPE. This research work examines the effect of pH in PBS, scan rate, and LF concentration on the surface of P(DLP/MCPE). The outcome result shows that the oxidation reaction of LF pH is an adsorption-controlled path. The detection limit of LF ranges from 0.2 μM to 7.0 μM, and the lower limits of detection (LOD) is 0.613x10⁻⁷ M and the lower limits of quantification (LOQ) is 2.051X10⁻⁷ M. The electrochemical P(DLP/MCPE) shows good repeatability, reproducibility, and stability. Finally, by analyzing LF in a pharmacological sample, the real time application of the sensor was assessed, and significant recoveries were observed.

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