Gesellschaft Deutscher Chemiker
Keine Benachrichtigungen
Sie haben noch keine Lesezeichen


Dye‐linked Upconverting Nanophosphor‐based Ratiometric Chemosensor for NIR‐excited and FRET‐mediated Ultrasensitive Detection of Sulfide Ions

Von Wiley-VCH zur Verfügung gestellt

Ratiometric chemosensor (UCNP@PAA-RBD-Sn2+) for FRET based S2− ions sensing following NIR light irradiation. This sensor and technique provides high selectivity and ultra-sensitivity towards the S2− ions, with a limit of detection (LOD) of 0.03 μM, which is far lower than WHO allowed contamination limit and previously reported probes for the same.


In this article, a NIR-excited ratiometric method for selective and ultrasensitive detection of sulfide (S2−) ions in an aqueous environment is presented, which is reliant on fluorescence resonance energy transfer (FRET). The chemosensor-design is based on an upconverting nanophosphor (UCNP)-linked coloured complex between a rhodamine-B derivative and stannous ions (RBD-Sn2+). Under the excitation of NIR light, the green emission of UCNP (at 542 nm) is reabsorbed by the linked RBD-Sn2+ complex via FRET, which in turn emits in the orange (at 582 nm). S2− ions specifically interact with the linked RBD-Sn2+ complex and quantitatively bleach its colour, leading to reduction in FRET and consequent recovery of the green emission of UCNP. We investigated the quantitative and ratiometric detection of S2− ions in aqueous phase using this NIR-excited FRET-based approach. The concentration of S2− ions is quantitatively connected to the ratiometric emission signal under NIR excitation. We have found that the detection limit for S2− ions using our FRET-based nanoprobe is around ten times lower than that of colorimetric or fluorescence-based approaches. Owing to the use of NIR-light as excitation source, our proposed sensor can demonstrate background-free sensing of S2− in complex environmental and biological samples.

Zum Volltext

Überprüfung Ihres Anmeldestatus ...

Wenn Sie ein registrierter Benutzer sind, zeigen wir in Kürze den vollständigen Artikel.