Minimally invasive boron neutron capture therapy (BNCT) is one of the elegant approaches for cancer treatment. The deliverability of boron agents to cancer cells with high selectivity and efficiency is the key to maximizing the therapeutic benefit...
A Fluorophore‐Labeled Lysine Dendrimer with an Oxo‐Anion‐Binding Motif for Tracking Gene Transfection
Von Wiley-VCH zur Verfügung gestellt
A transfection vector based on a peptide dendrimer (1) was developed and its ability for DNA binding and transport investigated. By attaching a fluorophore to the vector system (1*), several steps in the transfection process could be monitored directly. As DLS and AFM studies showed, the labeled vector1*condensed DNA into tightly packed aggregates able to enter eukaryotic cells. Co-localization experiments revealed that the ligand/plasmid complex is taken upviathe endosomal pathway followed by an endosomal escape or lysosomal degradation. Afterwards, the plasmid DNA seems to enter the nucleus due to a breakdown of the nuclear envelope during mitosis, as only cells that have recently undergone mitosis showed H2B-GFP expression.Zum Volltext
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