An electrochemical biosensor based on the supramolecular host-guest recognition between cucurbituril (CB) and L-phenylalanine-Cu(II) complex is reported for pyrophosphate (PPi) and alkaline phosphatase (ALP) analysis.
We report an electrochemical biosensor based on the supramolecular host-guest recognition between cucurbituril (CB) and L-phenylalanine-Cu(II) complex for pyrophosphate (PPi) and alkaline phosphatase (ALP) analysis. First, L-Phe-Cu(II) complex is simply synthesized by the complexation of Cu(II) (metal node) with L-Phe (bioorganic ligand), which can be immobilized onto CB modified electrode via host-guest interaction of CB and L-Phe. In this process, the signal of the complex-triggered electro-catalytic reduction of H2O2 can be captured. Next, due to the strong chelation between PPi and Cu(II), a biosensing system of the model “PPi and Cu(II) premixing, then adding L-Phe” was designed and the platform was applied to PPi analysis by hampering the formation of L-Phe-Cu(II) complex. Along with ALP introduction, PPi can be hydrolyzed to orthophosphate (Pi), where abundant Cu(II) ions are released to form L-Phe-Cu(II) complex, which gives rise to the catalytic reaction of complex to H2O2 reduction. The quantitative analysis of H2O2, PPi and ALP activity was achieved successfully and the detection of limits are 0.067 μM, 0.42 μM and 0.09 mU/mL (S/N=3), respectively. With its high sensitivity and selectivity, cost-effectiveness, and simplicity, our analytical system has great potential to for use in diagnosis and treatment of ALP-related diseases.Zum Volltext