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Rapid and Ultrasensitive Electrochemical Detection of TP53 Gene Mutation in Blood: Hybridization with a DNA/Gold‐Coated Magnetic Nanoparticle Network

Von Wiley-VCH zur Verfügung gestellt

A rapid and ultrasensitive electrochemical sensing method for the detection of TP53 gene containing a single-base mutation is reported in this work. This DNA biosensor can detect mutated TP53 gene directly in unprocessed blood samples using an electrically reconfigurable network of gold-coated magnetic nanoparticles with a broad dynamic range, high sensitivity and excellent selectivity.


Abstract

Mutant TP53 has been demonstrated to be a promising biomarker as it is the most frequently mutated gene in human cancer. However, quantifying TP53 mutation presents two unique challenges: a broad concentration range and ultralow abundance. This study reports on the development of an ultrasensitive and highly-selective electrochemical biosensor for the rapid detection of TP53 mutation in blood. TP53 mutation analysis is based on a network of gold-coated magnetic nanoparticles modified with probe DNA (DNA−Au@MNPs). The DNA−Au@MNPs biosensor can rapidly and selectively detect TP53 gene with a single-base mutation at a high background of wild-type TP53 gene in blood. Moreover, the sensor presents a broad dynamic range of 1 aM to 10 nM for the mutant TP53 gene with the lowest detected concentration of 1 aM and significantly reduced response time of 20 min. Therefore, this biosensor demonstrates the potential to be used for minimally invasive analysis of cancer.

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