QSAR‐Guided Study for the Microwave‐Assisted Synthesis of 4‐Methylquinoline Derivatives with Antimycobacterial Activity

To design new antituberculosis agents with a quinoline structure, our group developed three-dimensional structure-activity relationship (3D-QSAR) models, based on Comparative Molecular Field Analysis (CoMFA) and Comparative Molecular Similarity Index Analysis (CoMSIA). To validate the models, twelve quinolines through the Friëdlander reaction via microwave irradiation and three indolinones were synthesized. Additionaly, the compounds were evaluated in the growth inhibition of resistant H₃₇Rv tuberculosis strains (rpoB^S450L, katG^del, and gyrA^D94K) and in the nontuberculous strains Mycobacterium avium and M. abscessus. We found that the compound (Z)-4-((2-oxoindolin-3-ylidene)amino)-N-(thiazol-2-yl)benzenesulfonamide was active in all resistant strains; the compound 2-(1H-indol-3-yl)-4-methylquinoline was active on M. avium and the compound 10-methyl-11H-indeno[1,2-b]quinoline was active on M. abscessus.

Abstract

Tuberculosis was discovered more than a century ago, and it is still a disease that presents difficulties in its treatment due to the appearance of new resistant strains. To design new antituberculosis agents with a quinoline structure, our group developed three-dimensional structure-activity relationship (3D-QSAR) models, based on Comparative Molecular Field Analysis (CoMFA) and Comparative Molecular Similarity Index Analysis (CoMSIA). Statistically robust models (q²>0.6; r² _ncv>0.8; r² _pred>0.7) and with good external predictability were obtained. We found that positions 2, 3, and 4 of the quinoline nucleus are directly related to the modulation of the growth inhibitory activity of Mycobacterium tuberculosis H₃₇Rv. To validate the models, we synthesized twelve quinolines through the Friëdlander reaction and three indolinones. The synthesized compounds were evaluated in the growth inhibition of resistant H₃₇Rv tuberculosis strains (rpoB^S450L, katG^del, and gyrA^D94K) and in nontuberculous strains (M. avium and M. abscessus). We found that the compound (Z)-4-((2-oxoindolin-3-ylidene)amino)-N-(thiazol-2-yl)benzenesulfonamide was active in all resistant strains; the compound 2-(1H-indol-3-yl)-4-methylquinoline was active on M. avium; and the compound 10-methyl-11H-indeno[1,2-b]quinoline was active on M. abscessus. These models are useful for the discovery of new compounds with inhibitory properties of M. tuberculosis H₃₇Rv, and with potential applications in resistant and nontuberculous strains.

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