Photobioelectrocatalysis of Intact Photosynthetic Bacteria Exposed to Dinitrophenol

Purple bacteria photobioelectrocatalysis: 2,4-Dinitrophenol affects the photobioelectrocatalysis of biohybrid photoelectrodes based on intact photosynthetic bacteria paving the way for the development of biosensors for the early monitoring of this contaminant.

Abstract

The outstanding metabolic versatility of purple non-sulphur bacteria makes these organisms an ideal candidate for developing photobioelectrochemical systems applicable in contaminated environments. Here, the effects of 2,4 dinitrophenol, a common contaminant, on purple bacteria photobioelectrocatalysis were investigated. The aromatic contaminant clearly affects current generation, with an enhanced photocurrent obtained at low dinitrophenol concentrations (0.5–1 μM), while higher values (up to 100 μM) resulted in a gradual decrease of photocurrent. The obtained electrochemical evidence, coupled to spectroscopic studies, allowed verifying the viability of the bacteria cells after exposure to dinitrophenol, and that no alteration of the photosynthetic apparatus was obtained. The results indicate that high dinitrophenol concentrations divert electrons from the extracellular electron pathway to an alternative electron sink. The present results open the door to the possible use of intact bacteria-based photoelectrodes to develop technologies for sustainable biosensors with simultaneous environmental remediation.

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