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Fluorescent Probe for the Monitoring of Plasma Membrane Hydration

Von Wiley-VCH zur Verfügung gestellt

Two fluorescent probes are reported to detect plasma membrane hydration in cells under homeostatic and apoptotic conditions through an integrated dual-color bioanalytical protocol.


Abstract

Subcellular microenvironment plays a crucial role in maintaining cell homeostasis being hydration an essential parameter to monitor the variations of lipid organization in biological membranes. Fluorescent microenvironment-sensitive probes are stimuli-responsive molecules and able to change their fluorescence profile according to the microenvironment properties (polarity, hydration, microviscosity and tension). Here we present an organic fluorescent probe, NPM-1, as a new microenvironmental dye with selective plasma membrane localization in live cells. Using high-resolution confocal microscopy and fluorescence spectroscopy, we explored the hydration and polarity variations during the formation of apoptotic bodies in real time. To have a clear understanding of the cell microenvironment variations, an analogue polarity probe able to localize the cytosol and nucleoli was also synthetized, CellPol-1. Then, we were able to monitor the subcellular hydration variations during the apoptotic body dynamics.

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