Macrocyclization reactions are still challenging due to competing oligomerization, which requires the use of small substrate concentrations. Here, the cationic tungsten imido and tungsten oxo alkylidene N-heterocyclic carbene complexes [[W(N-2,6-C...
Artikel
Engineering an O‐methyltransferase for the Regioselective Biosynthesis of Hesperetin Dihydrochalcone
Von Wiley-VCH zur Verfügung gestellt
Engineering enhanced regioselectivity: Directed evolution of an O-methyltransferase resulted in variants with increased regioselectivity for the para-methylation of dihydrochalcones and related catecholic compounds (regioisomeric ratio up to 99 : 1). This allows now the biocatalytic production of the taste active hesperetin dihydrochalcone.
Abstract
Directed evolution of the O-methyltransferase ZgOMT from Zooshikella ganghwensis focusing on active site residues resulted in highly regioselective biocatalysts (regioisomeric ratios up to 99 : 1) for the preparation of the taste active hesperetin dihydrochalcone and related compounds. These newly constructed enzyme variants provide an attractive synthesis route for para-methylation of catechol scaffolds, which is challenging to perform with high regioselectivity utilizing wild-type O-methyltransferases.
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