BAMF0695, a self-sufficient CYP102 enzyme, hydroxylates sub-terminal positions of fatty acids with broad temperature optimum and high thermostability. Mutants of BAMF0695 exhibit very high regioselectivity, generating a single hydroxy fatty acid ...
Artikel
Chemical Synthesis of Peptides and Proteins Bearing Base‐Labile Post‐Translational Modifications: Evolution of the Methods in Four Decades
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The S-palmitoylation on Cys residue and O-acetylation on Ser/Thr residues are base-labile post-translational modifications. Their lability to bases and nucleophiles makes them challenging synthetic targets. In this review, we summarized the efforts toward their preparation during the past 40 years, with the focus on the evolution of synthetic methods.
Abstract
The S-palmitoylation on Cys residue and O-acetylation on Ser/Thr residues are two types of base-labile post-translational modifications (PTMs) in cells. The lability of these PTMs to bases and nucleophiles makes the peptides/proteins bearing S-palmitoyl or O-acetyl groups challenging synthetic targets, which cannot be prepared via the standard Fmoc-SPPS and native chemical ligation. In this review, we summarized the efforts towards their preparation in the past 40 years, with the focus on the evolution of synthetic methods.
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