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Applications of Terminal Deoxynucleotidyl Transferase Enzyme in Biotechnology

Von Wiley-VCH zur Verfügung gestellt

TThe use of polymerase enzymes in biotechnology has allowed us to gain unprecedented control over the manipulation of DNA, opening up new and exciting applications in areas such as biosensing, polynucleotide synthesis, and DNA storage aptamer development and DNA-nanotechnology. One of the most intriguing enzymes which has gained prominence in the last decade is terminal deoxynucleotidyl transferase (TdT), which is one of the only polymerase enzyme capable of the template independent stepwise addition of nucleotides onto an oligonucleotide chain.  This unique enzyme has seen a significant increase in a variety of different applications. In this review, we will give a comprehensive discussion of the unique properties and application of TdT as a biotechnology tool. We will discuss the application of TdT in the enzymatic synthesis of poly/oligonucleotides. Finally, we will look at the increasing role of TdT in biosensing, DNA storage, synthesis of DNA nanostructures and aptamer development, and give a future outlook for this technology.

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